Field inversion gel electrophoresis on Pseudomonas cepacia strains isolated from cystic fibrosis patients.
Genome fingerprinting by field inversion gel electrophoresis (FIGE) was utilized to typify 129 isolates of Pseudomonas cepacia (Pc) from 59 patients with cystic fibrosis (CF) and from environmental cultures in the CF ward.
The aim of this study was to assess whether a segregation policy avoided colonization of CF patients by nosocomial strains and contamination of the environment by colonized individuals, whether or not an'epidemic strain'was present in the ward and whether cross-colonization occurred in CF individuals subjected to prolonged close contact.
The Pc strains of each patient remained unchanged over time ; 78% of the genome finger-printings (GFP) were individual, whereas the others gave rise to 9 GFP groups.
A spirometer was probably contaminated by a newly colonized patient.
Adequate sanitary measures and avoidance of excessive promiscuity are helpful for limiting but are unable to eliminate Pc transmission in the CF ward.
Direct or indirect transmission, however seems, more frequent in CF patients in contacts outside the hospital.
Mots-clés Pascal : Mucoviscidose, Isolat clinique, Bactériose, Infection, Burkholderia cepacia, Bactérie, Méthode fingerprint, Carte génétique, Milieu hospitalier, Association morbide, Infection nosocomiale, Transmission, Equipement, Homme, Appareil respiratoire pathologie, Appareil digestif pathologie, Pancréas pathologie, Maladie héréditaire, Métabolisme pathologie, Biologie moléculaire, Génétique
Mots-clés Pascal anglais : Cystic fibrosis, Clinical isolate, Bacteriosis, Infection, Burkholderia cepacia, Bacteria, Fingerprint method, Genetic mapping, Hospital environment, Concomitant disease, Nosocomial infection, Transmission, Equipment, Human, Respiratory disease, Digestive diseases, Pancreatic disease, Genetic disease, Metabolic diseases, Molecular biology, Genetics
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0328291
Code Inist : 002B11D. Création : 10/04/1997.