Prevalence and characteristics of human and bovine verotoxigenic Escherichia coli strains isolated in Galicia (north-western Spain).
An epidemiological study was carried out to determine the incidence and the serotypes of verotoxigenic Escherichia coli (VTEC) that cause infections in Galicia (north-western Spain).
Although, VTEC strains were isolated from 55 (14%) of the 387 calves sampled and the majority of bovine VTEC strains belonged to serotypes (O26 : H11 or H-O91 : H21, O103 : H2, O105 : H18, O111 : H-O113 : H21, O126 : H-O128 : H-and O157 : H7 or H-) previously associated with human haemorrhagic colitis (HC) and haemolytic uraemic syndrome (HUS) in other countries, VTEC are not a common cause of human infections in Spain.
Thus, VTEC (O26 : H1 and O86 : H10) were isolated from only 3 (0.6%) of the 482 children with diarrhoea investigated.
We examined the 69 (3 humans and 66 bovines) VTEC strains that were initially isolated as E. coli producing a toxin cytotoxic to Vero and HeLa cells by polymerase chain reaction (PCR) using specific primers for VT1, VT2 and eae genes.
PCR showed that 38 (55%) of VTEC strains carried VTI genes, 18 (26%) possessed VT2 genes, and 10 (14%) carried both VT1 and VT2 genes.
Three (one human and two bovine) strains which were formerly VTEC had lost the ability to produce verotoxins upon subculture and became negative for VT1 and VT2 by PCR.
In total 35 (51%) of 69 VTEC strains, including the two human VT1+strains of serotype O26 : H11, were positive for eae sequences when tested by PCR. (...)
Mots-clés Pascal : Entérotoxine, Origine microbienne, Escherichia coli, Enterobacteriaceae, Bactérie, Sérotypie, Epidémiologie, Diarrhée, Espagne, Europe, Homme, Bovin, Artiodactyla, Ungulata, Mammalia, Vertebrata, Pathogénie, Appareil digestif pathologie, Intestin pathologie, Vérotoxine
Mots-clés Pascal anglais : Enterotoxin, Microbial origin, Escherichia coli, Enterobacteriaceae, Bacteria, Serotyping, Epidemiology, Diarrhea, Spain, Europe, Human, Bovine, Artiodactyla, Ungulata, Mammalia, Vertebrata, Pathogenesis, Digestive diseases, Intestinal disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0324548
Code Inist : 002A05B11. Création : 10/04/1997.