Detection of microcystins, a blue-green algal hepatotoxin, in drinking water sampled in Haimen and Fusui, endemic areas of primary liver cancer in China, by highly sensitive immunoassay.
An epidemiological survey for the causes of a high incidence of primary liver cancer (PLC) in Haimen city, Jian-Su province and Fusui county, Guangxi province in China, found a close correlation between the incidence of PLC and the drinking of pond and ditch water.
With an aim to clarify whether microcystins (MC), a hepatotoxic peptide produced by water bloom algae, contaminate the drinking water in the endemic areas of PLC in China, a highly sensitive enzyme-linked immunosorbent assay with a detection limit of 50 pg/ml, was introduced to monitor the MC.
Three trials to survey the drinking water were carried out in 1993-1994.
Samples, 1135 in total, were collected from different sources such as : ponds, ditches, rivers, shallow wells and deep wells in Haimen city.
The first survey in September 1993 found that three out of 14 ditch water specimens were positive for MC, with a range of 90-460 pg/ml.
Several toxic algae such as Oscillatoria agardhii were present in some of the ditches.
In the second trial, samples were collected from five ponds/ditches, two rivers, two shallow wells and two deep wells monthly for the whole year of 1994.
These data showed that MC was highest in June to September, with a range of 62-296 pg/ml.
A third trial on the 989 different water samples collected from the different types of water sources in July 1994 revealed that 17% of the pond/ditch water, 32% of the river water, and 4% of the shallow-well water were positive for MC, wi...
Mots-clés Pascal : Oscillatoria, Cyanobacteria, Bactérie, Toxine, Eau potable, Tumeur maligne, Chine, Asie, Epidémiologie, Homme, Détection, Technique ELISA, Méthode immunoenzymatique, Analyse quantitative, Carcinogène, Variation saisonnière, Appareil digestif pathologie, Foie pathologie, Oscillatoria agardhii, Microcystine
Mots-clés Pascal anglais : Oscillatoria, Cyanobacteria, Bacteria, Toxin, Drinking water, Malignant tumor, China, Asia, Epidemiology, Human, Detection, ELISA assay, Enzyme immunoassay, Quantitative analysis, Carcinogen, Seasonal variation, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0299915
Code Inist : 002B13C01. Création : 199608.