The lymphocyte bulky PAH-DNA adduct levels have been studied in persons occupationally exposed to ambient air pollution.
The exposure group consisted of 90 healthy, non-smoking bus drivers from the Copenhagen area, divided into three exposure groups according to driving area, and 60 rural controls (smokers and non-smokers).
PAH-DNA adducts were determined by 32P-postlabelling with the butanol enrichment procedure.
The bus drivers answered a comprehensive questionnaire on passive smoking, residential area, diet and other potential confounding variables.
A significantly higher adduct level was observed in bus drivers working in central Copenhagen (1.214 fmol/mug DNA, n=49) compared with both those driving in the dormitory (median : 0.507 fmol/mug DNA, P=0.046, n=16) and suburban (median : 0.585 fmol/mug DNA, P=0.041, n=25) areas.
All three groups had higher adduct levels than rural controls (0.074 fmol/mug DNA, n=60, P<0.001).
No significant influence on adduct levels was demonstrated from potential confounders, including smoking and diet.
The effect of the metabolizing enzymes, GSTMI and NAT2, on adduct levels was investigated.
No statistically significant effects were observed on adduct levels from GSTMI or NAT2, either individually or combined, but a non-significant trend was seen for individuals with GSTM1*0/0 (null), since they had higher adduct levels in all exposure groups.
This study demonstrated that lymphocyte PAH-DNA adduct levels were related to ...
Mots-clés Pascal : Pollution air, Milieu urbain, Conducteur véhicule, Autobus, Exposition professionnelle, Médecine travail, Homme, Danemark, Europe, Adduit moléculaire, DNA, Composé aromatique polycyclique, Etude comparative, Zone urbaine, Zone suburbaine, Zone rurale, Glutathione transferase, Transferases, Enzyme, Arylamine N-acetyltransferase, Acyltransferases, Génotype, Lymphocyte, Cellule sanguine, Drug-metabolizing enzyme, Zone dortoir
Mots-clés Pascal anglais : Air pollution, Urban environment, Vehicle driver, Bus, Occupational exposure, Occupational medicine, Human, Denmark, Europe, Molecular adduct, DNA, Polycyclic aromatic compound, Comparative study, Urban area, Suburban zone, Rural area, Glutathione transferase, Transferases, Enzyme, Arylamine N-acetyltransferase, Acyltransferases, Genotype, Lymphocyte, Blood cell, Drug-metabolizing enzyme
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0271624
Code Inist : 002B03M02. Création : 199608.