To evaluate in a large group of volunteer blood donors the prevalence of antibodies to hepatitis C virus (anti-HCV) and the relation of transaminase (ALT) levels and viraemia to liver damage.
A prospective study.
Transfusion Centre of the Autonomous Community of Madrid and the Liver Unit of the Princesa University Hospital.
From a population of 55 587 volunteer blood donors, 160 seropositive cases were further evaluated for virological and histological assessment.
Anti-HCV was tested by ELISA-2 and RIBA-2 assays.
HCV RNA was analysed by nested PCR.
Liver biopsies were obtained in 35 volunteer blood donors with abnormal ALT levels.
The prevalence of anti-HCV detected by ELISA-2 was 0.93%. Serum ALT was abnormal in 61 of the 160 volunteers (38.1%). Of these, RIBA-2 was positive in 96.7% and HCV RNA was detectable in 96.1%. Serum ALT was normal in the remaining 99,70.7% being RIBA-2 negative and 98.3% HCV RNA negative.
The majority of biopsies (85.6%) showed chronic hepatitis.
This study demonstrates that in blood donors screening for anti-HCV, a positive ELISA-2 test, when associated with abnormal ALT levels, is effective in recognizing subjects with active infection detected by HCV RNA and liver disease.
Concerning ELISA-2 positive volunteer blood donors with normal ALT, long-term studies are warranted to elucidate whether they are really infected by HCV.
Mots-clés Pascal : Hépatite virale C, Virose, Infection, Donneur sang, RNA, Anticorps, Epidémiologie, Facteur risque, Prévalence, Homme, Espagnol, Transfusion, Appareil digestif pathologie, Foie pathologie
Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Blood donor, RNA, Antibody, Epidemiology, Risk factor, Prevalence, Human, Spanish, Transfusion, Digestive diseases, Hepatic disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0271176
Code Inist : 002B30A01C. Création : 199608.