Use of IS6110 DNA fingerprinting in tracing man-to-man transmission of Mycobacterium tuberculosis in the Czech Republic.
The epidemiological relationship between tuberculosis cases in a prison and between cases within five families was investigated.
Therefore, the isolated Mycobacterium tuberculosis strains were subjected to restriction fragment length polymorphism (RFLP) analysis using insertion sequence IS6110 as a probe.
In case of 1 patients, the expected links of transmission were confirmed by RFLP typing.
In contrast, in case of 4 patients the conclusion of classical contact tracing were not in agreement with the DNA fingerprinting results.
These findings reinforce the usefulness of this recently developed technique as an additional tool in contact tracing.
The IS6110DNA fingerprints of all strains investigated consisted of 7 to 13 bands and showed a high degree of polymorphism.
Comparison of these fingerprints with those recorded in the Czech Republic previously, revealed the presence of a predominant DNA fingerprint types, without a known connection between the cases.
Futhermore, other patterns found in the present study showed a high degree of similarity with the previously obtained fingerprints.
Part of the patients were sampled twice.
All of these double isolates showed identical fingerprints, confirming the previously described stability of IS6110DNA fingerprints.
In contrast, from one couple of strains, isolated from husband and wife both suffering from tuberculosis, a slight change in one of the two patterns was observed.
The patterns shared 10 band positions, ...
Mots-clés Pascal : Tuberculose, Mycobactériose, Bactériose, Infection, Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bactérie, Transmission homme homme, Milieu familial, Milieu carcéral, Epidémiologie, Polymorphisme longueur fragment restriction, Marqueur génétique, Méthode fingerprint, Isolat clinique, Homme, République tchèque, Europe, Infection communautaire
Mots-clés Pascal anglais : Tuberculosis, Mycobacterial infection, Bacteriosis, Infection, Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bacteria, Transmission from man to man, Family environment, Carceral environment, Epidemiology, Restriction fragment length polymorphism, Genetic marker, Fingerprint method, Clinical isolate, Human, Czech Republic, Europe, Community infection
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0267497
Code Inist : 002B05B02E. Création : 199608.