Hypersensitivity reactions and specific antibodies in workers exposed to industrial enzymes at a biotechnology plant.
Thirty-six employees who produced industrial enzymes from selected strains of bacteria and fungi were evaluated by epicutaneous threshold testing and enzyme-linked immunosorbent assays (ELISA) for specific IgE and IgG antibodies.
The workers complained of'asthma-and flu-like'symptoms, which generally lessened away from work.
The enzymes evaluated were :
alpha-amylase (1,4-alpha-d. glucan glucanohydrolase) from Bacillus licheniformis (alphaABl),
B. subtilis formation 1 (alphaAlBs) and B. subtilis formation 2 (alphaA2Bs) ;
purified alpha-amylase from B. licheniformis (CalphaABl) and A. oryzae (CalphaAAo) ;
alkaline protease from B. licheniformis (APBl) and purified alkaline protease (CAPBl) ;
amyloglucosidase (1,4-alpha-d-glucan glucohydrolase) from A. niger (AGAn) and purified amyloglucosidase (CAGAn).
Statistically significant increases (P>0.05) in the proportion of workers having positive skin tests to CAPBl, AGAn and CAGAn were found.
Significantly elevated (P>0.05) mean specific IgE results were observed for CalphaAAo CAGAn and AGAn, and elevated (P>0.05) mean specific IgGs were observed for CalphaAAo, CAGAn, AGAn, alphaAlBs, alphaABI and alphaA2Bs.
These results indicate that occupational exposure to some industrial enzymes can cause immediate-onset cutaneous hypersensitivity reactions, pulmonary function deficits and significantly elevated specific antibody levels.
Our results are equivocal as to whether work-related respiratory and cutaneous hypersen...
Mots-clés Pascal : Enzyme, Fonction respiratoire, Génie génétique, Biotechnologie, Industrie, Exposition professionnelle, Médecine travail, Toxicité, Homme, Hypersensibilité, Technique ELISA, IgE, IgG, Allergie, Asthme, Immunopathologie, Appareil respiratoire pathologie, Test cutané
Mots-clés Pascal anglais : Enzyme, Lung function, Genetic engineering, Biotechnology, Industry, Occupational exposure, Occupational medicine, Toxicity, Human, Hypersensitivity, ELISA assay, IgE, IgG, Allergy, Asthma, Immunopathology, Respiratory disease, Skin test
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0224239
Code Inist : 002B03O. Création : 199608.