A method for assessing the viability of nematode eggs in sludge.
Helminth eggs are found in urban sludge used in agriculture.
Then, to evaluate the parasitic risk, a method was developed to estimate the viability of these eggs.
The extraction of these eggs was performed with a diphasic treatment (SDS 0.01% - Ethylacetate) coupled with a filtration on 500 mum and 100 mum sieves followed by concentration using two flotations with NaCI (d=1.19).
For the culture, 3 parameters tested showed a faster egg development at 30°C in deionized water with continuous aeration, whereas organic compounds reduced this development.
This culture was performed during respectively 13,10 and 8 days to obtain Ascaris suum, Toxocara canis and Capillaria sp larva, and 16 days for Trichuris vulpis which presented a slower development.
The quantification was realized by microscopic examination (100 X) after sonication and sodium hypochlorite (0.5° Chl) treatment.
The eggs reaching the larva stage were considered as viable and the percent of viability was expressed by the ratio : nematode eggs at the larva stage/total of Nematode eggs counted.
Mots-clés Pascal : Viabilité, Oeuf, Boue résiduaire, Déchet urbain, Epidémiologie, Nématodose, Helminthiase, Parasitose, Infection, Amendement organique, Pollution sol, Ascaris suum, Nematoda, Nemathelminthia, Helmintha, Invertebrata, Toxocara canis, Parasite, Ecotoxicologie, Pathogène, Capillaria, Trichuris vulpis
Mots-clés Pascal anglais : Viability, Egg, Sewage sludge, Urban waste, Epidemiology, Nematode disease, Helminthiasis, Parasitosis, Infection, Organic amendment, Soil pollution, Ascaris suum, Nematoda, Nemathelminthia, Helmintha, Invertebrata, Toxocara canis, Parasite, Ecotoxicology, Pathogenic
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0218840
Code Inist : 002A14B03C1. Création : 199608.