Recently, a trinucleotide repeat polymorphism at the rad (ras associated with diabetes) locus (RAD1) on chromosome 16q was described in association with NIDDM in white Americans.
In an attempt to replicate this finding, we screened RAD1 and another microsatellite marker at the D16S265 loci, which is located near the rad locus, with a radioactive polymerase chain reaction method in 290 unrelated Finnish NIDDM patients and 270 control subjects and related the findings to measures of insulin sensitivity.
Both groups were randomly selected from the western (189 NIDDM patients and 184 control subjects) and southern (101 NIDDM patients and 86 control subjects) parts of Finland.
The allele frequency distributions of RAD1 and D16S265 did not differ between NIDDM patients and control subjects in the studied population groups.
The genotype distribution was also analyzed by structural classes of the RAD1 polymorphism, and no difference was detected between the NIDDM and control groups.
In addition, carriers of allele classes I, II, and IV (reported to be preferentially associated with NIDDM in white Americans) did not differ from the class III homozygotes with respect to age at onset of NIDDM, BMI, or rates of insulin-stimulated glucose disposal.
In conclusion, we found no association between the rad locus and NIDDM or insulin resistance in Finnish NIDDM patients.
Mots-clés Pascal : Diabète non insulinodépendant, Résistance tissu cible, Insuline, Déterminisme génétique, Marqueur génétique, Polymorphisme, Endocrinopathie, Epidémiologie, Homme, Finlande, Europe, Gène rad
Mots-clés Pascal anglais : Non insulin dependent diabetes, Target tissue resistance, Insulin, Genetic inheritance, Genetic marker, Polymorphism, Endocrinopathy, Epidemiology, Human, Finland, Europe
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0200571
Code Inist : 002B21E01A. Création : 199608.