Objectives-The pathogenic basis of respiratory disorders associated with isocyanates are still obscure.
One reason for this is the lack of good estimates of human exposure.
In this study exposure was estimated by measurement of isocyanate metabolites in biological samples.
Methods-In a factory using isocyanate based polyurethane (PUR) glue, isocyanate concentrations in air were measured by liquid chromatography.
Samples from 174 employees were analysed for metabolites of 4,4'-methylene diphenyl diisocyanate (MDI) in plasma (P-MDX) and urine (U-MDX).
After hydrolysis, 4,4'-methylenedianiline was measured by gas chromatography-mass spectrometry (GC-MS).
The employees were screened for work related respiratory symptoms and tested for specific immunoglobulin E (IgE) and IgG antibodies directed against isocyanate conjugated to human serum albumin.
The time weighted isocyanate concentrations in air were low (MDI<0.2-7 ; hexamethylene diisocyanate (HDI)<0.1-0.7 ; 2,6-toluene diisocyanate (TDI)<0.1 mug/m3).
All subjects had detectable P-MDX and U-MDX.
There were significant associations between the estimates of exposure to thermal degradation products of an MDI based glue and P-MDX (range ¾ 0.10-5.5 mug/l), and U-MDX (¾ 0.04-5.0 mug/g creatinine) ; in cases of heavy exposure.
P-MDX and U-MDX were associated with each other (r=0.64 ; P=0.0001), work related symptoms (P-MDX : P=0.03 ; Mann-Whitney U test), and serum concentrations of MDI specific IgG antibod...
Mots-clés Pascal : Adhésif, Uréthanne polymère, Industrie, Isocyanate organique, Toxicité, Homme, Exposition professionnelle, Médecine travail, Surveillance biologique, Marqueur biologique, Urine, Plasma sanguin, Fonction hépatique, Anticorps, Méthode immunologique, Diphénylméthane(4,4p-diisocyanato)
Mots-clés Pascal anglais : Adhesive, Polyurethane, Industry, Organic isocyanate, Toxicity, Human, Occupational exposure, Occupational medicine, Biological monitoring, Biological marker, Urine, Blood plasma, Liver function, Antibody, Immunological method
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0199752
Code Inist : 002B03L06. Création : 199608.