Vibrio vulnificus is a potentially lethal human pathogen capable of producing septicemia in susceptible persons.
Disease is almost always associated with consumption of seafood, particularly raw oysters, or with exposure of wounds to seawater.
An oligonucleotide DNA probe (V. vulnificus alkaline phosphatase-labeled DNA probe epsilonVVAPzêta), previously shown to be highly specific for V. vulnificus, was used to enumerate this species in environmental samples collected from the Chesapeake Bay between April 1991 and December 1992.
Total aerobic, heterotrophic, culturable bacteria were enumerated by plate counts on nonselective medium.
The number of V. vulnificus organisms was determined by colony lifts of spread plates for subsequent hybridization with VVAP.
V. vulnificus was not detected in any samples collected during February and March (water temperature of<8\C) but was found in 80% of the water samples collected during May, July, September, and December (water temperature of>8\C), with concentrations ranging from 3.0 x 10#F1 to 2.1 x 10#F2/ml (ca. 8% of the total culturable heterotrophic bacteria).
In a multiple regression analysis, increased V. vulnificus concentrations were correlated with lower salinities and with isolation from samples collected closer to the bottom.
Isolation from oysters was demonstrable when water temperatures were 7.6\C, with concentrations ranging from 1.0 x 10#F3 to 4.7 x 10#F4/g (ca. 12% of total culturable bacteria).
Mots-clés Pascal : Vibrio vulnificus, Vibrionaceae, Bactérie, Pathogène, Milieu marin, Dénombrement, Sonde moléculaire, Milieu culture, Huître, Bivalvia, Mollusca, Invertebrata, Température, Plancton, Salinité, Sédiment, Eau mer, Santé publique, Facteur milieu, Baie Chesapeake
Mots-clés Pascal anglais : Vibrio vulnificus, Vibrionaceae, Bacteria, Pathogenic, Marine environment, Enumeration(counting), Molecular probe, Culture medium, Oyster, Bivalvia, Mollusca, Invertebrata, Temperature, Plankton, Salinity, Sediments, Seawater, Environmental factor
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0151618
Code Inist : 002A05B10. Création : 199608.