Primary DNA damage in peripheral mononuclear blood cells of workers exposed to bitumen-based products.
The genotoxic effect of occupational exposure to bitumen-based products was determined by the extent of DNA strand breaks and alkali-labile sites of the DNA of peripheral mononuclear blood cells from seven roofers, 18 road paving workers, and nine bitumen painters.
In order to evaluate short-term genotoxic effect the workers were investigated on Fridays and on Mondays after a weekend free of occupational exposure.
The roofers (all cigarette smokers) showed a significantly (P<0.002) 43% higher mean level of alkaline DNA strand breaks on Friday than did the ten smoking controls included in this study.
Also, comparison of the individual levels of alkaline strand breaks on Mondays and on Fridays revealed a significant increase (P<0.05, Wilcoxon test) during the work week.
In the road paving workers and the bitumen painters no statistically significant difference in the mean levels of alkaline strand breaks could be found compared to controls either for the measurement on Mondays or for that on Fridays.
However, interesting tendencies were observed.
As in the group of roofers, the mean level of alkaline DNA strand breaks as well as the majority of the individual levels of alkaline strand breaks of road paving workers was higher on Fridays than on Mondays.
In contrast, bitumen painters exhibited a relatively high level of alkaline DNA strand breaks on Mondays and a decreased mean level of strand breaks on Fridays.
DNA adducts could be detected at a low level (up...
Mots-clés Pascal : Bitume, Toxicité, DNA, Exposition professionnelle, Couvreur, Peintre en bâtiment, Construction routière, Bitume routier, Homme, Cassure bicaténaire, Adduit moléculaire, Surveillance biologique, Carcinogène, Mutagène, Médecine travail
Mots-clés Pascal anglais : Bitumen, Toxicity, DNA, Occupational exposure, Roofer, House painter, Road construction, Road bitumen, Human, Double strand break, Molecular adduct, Biological monitoring, Carcinogen, Mutagen, Occupational medicine
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0148432
Code Inist : 002B03N. Création : 199608.