Belgian Environmental Mutagen Society BEMS. Annual meeting. Mol BEL, 1994/12/07.
This paper shows some of the results that were recorded in our attempt to unambiguously identify and quantify the major EtO-DNA adduct, N#B7 (2 hydroxyethyl) guanine.
The use of mass spectrometry was found necessary in order to have a precise molecular determination of the adduct coupled to a sensitive dosimetry method.
Previous adduct isolation from the unmodified DNA units was investigated by the two separation methods usually coupled to a mass spectrometer : gas chromatography (GC/MS and GC/MS/MS) and high performance liquid chromatography (HPLC/MS).
GC/MS and GC/MS/MS were found unappropriate for a rigourous quantitative analysis ; this was probably due to a lack of sensitivity inherent to the method and to a lack of reproducibility coming from the derivatisation reaction.
HPLC/MS using electrospray ionisation was found appropriate for our purpose with the obtention of linear calibration curve up to 50 femtomoles of adduct.
This is in good agreement with the detection limit exigences usually related to cancer risk assessment after EtO exposure.
Mots-clés Pascal : Mutagène, Oxirane, Analyse quantitative, Adduit moléculaire, Chromatographie phase gazeuse, Spectrométrie masse, Chromatographie HPLC, Electrospray, Santé publique
Mots-clés Pascal anglais : Mutagen, Ethylene oxide, Quantitative analysis, Molecular adduct, Gas chromatography, Mass spectrometry, HPLC chromatography, Electrospray
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0144838
Code Inist : 002B03N. Création : 199608.