Immune reactivity of cow-asthmatic dairy farmers to the major allergen of cow (BDA20) and to other cow-derived proteins. The use of purified BDA20 increases the performance of diagnostic tests in respiratory cow allergy.
Background Cow dust is one of the most important inducers of occupational allergic diseases in Finland.
For example, in 1991 it accounted for almost 40% of the new occupational asthma cases.
Objective This study compares the performance of the purified major cow allergen (BDA20) and crude bovine epithelial extract (BEA) in diagnostic tests and examines the role of milk allergy-associated bovine proteins (bovine serum albumin, alpha-lactalbumin, bêta-lactoglobulin, casein) in respiratory cow allergy.
Methods The humoral responses of cow-asthmatic and healthy farmers to the various components of BEA were analysed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting.
The levels of specific IgE and IgG antibodies were quantificated with enzyme-linked immunosorbent assays (ELISAs).
The cellular responses were analysed with antigen-specific lymphocyte proliferation tests.
Results The specific anti-BDA20 IgE measurement was found to be best in distinguishing between the asthmatic farmers and their healthy colleagues.
It proved possible to determine a cut-off value that gave the analysis a specificity and sensitivity of 100% ; the distinction between the two groups was highly significant (P<0.0001).
In the lymphocyte proliferation analysis, cow asthma was more closely associated with reactivity to BDA20 than to BEA.
In the measurement of anti-BDA20 and anti-BEA IgG antibody levels, considerable overlap between the groups was...
Mots-clés Pascal : Asthme, Réponse immune, Immunité humorale, Allergie, Allergène, Epithélium, Phanère, Vache, Boeuf, Artiodactyla, Ungulata, Mammalia, Vertebrata, Animal, Exposition professionnelle, Protéine, Lait vache, Méthode immunoblotting, Electrophorèse gel, Diagnostic, Exploration, Agriculteur, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Immunopathologie, Maladie professionnelle, Médecine travail, Biologie moléculaire, Test prolifération lymphocyte
Mots-clés Pascal anglais : Asthma, Immune response, Humoral immunity, Allergy, Allergen, Epithelium, Dermoskeleton, Cow, Ox, Artiodactyla, Ungulata, Mammalia, Vertebrata, Animal, Occupational exposure, Proteins, Cow milk, Immunoblotting assay, Gel electrophoresis, Diagnosis, Exploration, Farmer, Human, Respiratory disease, Obstructive pulmonary disease, Immunopathology, Occupational disease, Occupational medicine, Molecular biology
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0119250
Code Inist : 002B06C02. Création : 199608.