Wheat antigen exposure assessment for epidemiological studies in bakeries using personal dust sampling and inhibition ELISA.
Background Asthma in bakery workers caused by exposure to wheat flour proteins is an important occupational health problem.
Until recently, gravimetric dust measurements were the only available technique for quantitative exposure assessment in bakeries.
However, it is questionable whether dust levels are a good exposure parameter or only give a crude approximation of the actual flour allergen concentration.
Objective In the present study we have investigated a method to measure wheat flour antigens with immunochemical methods.
Methods Wheat flour antigens were measured in 449 personal dust samples taken in bakeries, using enzyme-linked immunosorbent assay (ELISA) inhibition and an anti-wheat IgG4 serum pool.
Western-blotting was performed to compare the wheat flour proteins detected by IgE and IgG4.
Results Electrophoresis and immunoblotting showed that many wheat flour proteins can bind IgG4 and IgE, but also a reasonable similarity in major allergens detected by our IgG4-serum pool and IgE-positive sera.
Inhibition tests showed some cross-reactivity with some cereal species, but not with other ingredients used in bakeries.
In bakeries, large differences in personal airborne flour levels were found between occupational titles.
For several groups clear differences in wheat antigen exposure levels existed, where no differences in dust exposure levels could be found.
The relationship between dust and wheat antigen exposure varied considerably, depending on t...
Mots-clés Pascal : Asthme, Allergie, Exposition professionnelle, Farine blé, Allergène, Boulangerie, Echantillonnage, Technique ELISA, Méthode mesure, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Immunopathologie, Maladie professionnelle, Médecine travail, Méthode immunoenzymatique
Mots-clés Pascal anglais : Asthma, Allergy, Occupational exposure, Wheat flour, Allergen, Bakery, Sampling, ELISA assay, Measurement method, Human, Respiratory disease, Obstructive pulmonary disease, Immunopathology, Occupational disease, Occupational medicine, Enzyme immunoassay
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0113416
Code Inist : 002B06C02. Création : 199608.