Enzyme immunoassays for total and allergen specific IgE in population studies.
Extensive IgE serology in occupational or environmental health studies is often hampered by a lack of technical facilities and finance.
The use in population studies of relatively simple and inexpensive enzyme immunoassays (EIAs) was therefore evaluated for the assessment of total serum immunoglobulin E (IgE), and of specific IgE reactions with various common (house dust mites, grass and birch pollen, and cat) or occupational (fungal alpha-amylase and rat urinary protein) allergens.
Method-Total IgE was measured with a sandwich EIA, calibrated with commercially available IgE standards.
Reproducibility was studied by testing pooled normal human serum samples in each of a large series of test plates.
A panel of 156 children's serum samples with known IgE values was used to compare the assay with other total IgE assays.
A previously developed EIA for anti-yeast IgE was adapted for the measurement of IgE reacting with various common and occupational allergens.
Binding of IgE to microwells coated with commercially available allergen extracts, or allergen preparations from our own laboratory, was measured with a monoclonal antihuman IgE antibody and subsequent incubations with biotinylated rabbit anti-mouse Ig and avidin-peroxidase.
Panels of serum samples from school children (n=116), bakery workers (n=126), and laboratory animal workers (n=52) were used to study sensitivity and specificity, with reference to skin prick tests as the standard, and to c...
Mots-clés Pascal : Méthode immunoenzymatique, Analyse quantitative, IgE, Atopie, Allergie, Homme, Epidémiologie, Méthode étude, Sérum, Allergène, Exposition professionnelle, Médecine travail, Pays Bas, Europe, Immunopathologie
Mots-clés Pascal anglais : Enzyme immunoassay, Quantitative analysis, IgE, Atopy, Allergy, Human, Epidemiology, Investigation method, Serum, Allergen, Occupational exposure, Occupational medicine, Netherlands, Europe, Immunopathology
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0086202
Code Inist : 002B06E04. Création : 199608.