The objective of this study was to determine the prevalence and time of appearance of serum anti-p53 antibodies during the pathogenesis of ASL associated with occupational exposure to vinyl chloride.
Enzyme-linked immunoassay (EIA) was used to detect anti-p53 antibodies in 148 serum samples from 92 individuals occupationally exposed (in France or in Kentucky) to vinyl chloride ; 15 of these individuals (six from France and nine from Kentucky) had ASL.
A subset of coded EIA-positive and EIA-negative sera was further analyzed for anti-p53 antibodies by immunoblotting and immunoprecipitation.
Nucleotide sequence analysis of exons 5-8 of the p53 gene was conducted on ASL DNA from six patients.
We tested sera from 31 men who had no occupational exposure to vinyl chloride ; they made up the control group.
Statistical analyses were done using the Kruskal-Wallis chisquared approximation and the Wilcoxon two-sample test for normal approximation.
All P values result from two-sided tests.
Fourteen serum samples (from nine individuals) were positive in the EIA.
Serum anti-p53 antibodies can predate clinical diagnosis of certain tumors, such as ASL, and may be useful in identifying individuals at high cancer risk, such as workers with occupational exposure to vinyl chloride.
Mots-clés Pascal : Angiosarcome, Foie, Exposition professionnelle, Vinylique chlorure polymère, Carcinogène, Anticorps, Gène suppresseur tumeur, Pathogénie, Homme, Sarcome, Appareil circulatoire pathologie, Vaisseau sanguin pathologie, Tumeur maligne, Foie pathologie, Appareil digestif pathologie, Médecine travail
Mots-clés Pascal anglais : Angiosarcoma, Liver, Occupational exposure, Polyvinyl chloride, Carcinogen, Antibody, Tumor suppressor gene, Pathogenesis, Human, Sarcoma, Cardiovascular disease, Vascular disease, Malignant tumor, Hepatic disease, Digestive diseases, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0007314
Code Inist : 002B13C01. Création : 01/03/1996.