Diagnostic validation of specific IgE antibody concentrations, skin prick testing, and challenge tests in chemical workers with symptoms of sensitivity to different anhydrides.
The diagnostic possibilities of sensitization to various acid anhydrides are limited because of the lack of standardized allergens for the different test systems.
This makes the diagnosis of IgE-mediated sensitization caused by occupational exposure difficult.
We prepared conjugates of human serum albumin with phthalic, maleic, trimellitic, and pyromellitic anhydrides to be used for IgE estimation by enzyme-allergosorbent test, skin prick tests, and nasal and bronchial challenge tests.
Nine anhydride workers, who complained of various respiratory symptoms, were studied.
Of the nine workers, four had immediate-type skin test responses to one or more conjugates.
All four subjects had elevated IgE concentrations in addition to two other workers.
Three of six nasal challenges and four of nine bronchial challenges resulted in positive responses.
All but one of the positive nasal or bronchial test responses were associated with elevated IgE levels.
The seven positive challenge test results included five positive skin test responses.
On the other hand, in all but two of the subjects with negative challenge test results, no specific IgE could be detected.
In these two subjects the negative results were associated with low levels of IgE, and in one, with the absence of asthma.
None of the results of tests with unconjugated anhydrides were positive.
Mots-clés Pascal : Asthme, Allergie, Anhydride organique, Test cutané, Composé chimique, Composé organique, Méthode immunoenzymatique, IgE, Anticorps, Test provocation, Bronche, Immunoglobuline, Exposition professionnelle, Exploration immunologique, Diagnostic, Toxicité, Etiologie, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Immunopathologie, Médecine travail, Maladie professionnelle, Prick test
Mots-clés Pascal anglais : Asthma, Allergy, Organic anhydride, Skin test, Chemical compound, Organic compounds, Enzyme immunoassay, IgE, Antibody, Provocation test, Bronchus, Immunoglobulins, Occupational exposure, Immunological investigation, Diagnosis, Toxicity, Etiology, Human, Respiratory disease, Obstructive pulmonary disease, Immunopathology, Occupational medicine, Occupational disease, Prick test
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0592371
Code Inist : 002B06C02. Création : 01/03/1996.