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  1. Evaluation of the World Health Organisation antibody-testing strategy for the individual patient diagnosis of HIV infection (strategy III).

    Article - En anglais

    Objective

    To evaluate the World Health Organisation (WHO) antibody testing strategy for the individual patient diagnosis of HIV infection (strategy III).

    Design

    Evaluation of a combination of enzyme-linked immunosorbent assays (ELISAs) for the detection of antibodies to HIV-1 and HIV-2 infection.

    The WHO strategy III calls for a combination of three ELISAs, based on different antigens and/or differing test principles, to be used in a sequential fashion.

    The first part of the study evaluated various kits as part of a selection process.

    The second part of the study was an assessment of the three-ELISA testing strategy on routine sera submitted to the National Institute for Virology (NIV) for HIV testing.

    In all instances, the conventional testing protocol, which utilises a screening ELISA followed by a confirmatory Western blot (WB) on positive specimens, was used as the'gold standard'Setting.

    The HIV-testing laboratory at the NIV, Johannesburg.

    Results

    In the first part of the study, all of the ELISA kits evaluated showed high sensitivity and specificity, and a selection of the kits for part two of the study was made in accordance with the WHO recommendation.

    The kits selected, in order of use, were the Biotest anti-HIV 1/2 (test 1), Pasteur Genelavia Mixt HIV-1/2 (test 2) and Murex Wellcozyme HIV-1 competitive assay (test 3).

    This combination was evaluated using 7 812 sera submitted to the NIV for serology testing.

    Mots-clés Pascal : SIDA, Virose, Infection, Virus HIV1, Virus immunodéficience humaine, Lentivirinae, Retroviridae, Virus, Virus HIV2, Evaluation, Technique ELISA, Anticorps, OMS, Homme, République Sud Africaine, Afrique, Immunopathologie, Immunodéficit, Protocole diagnostic

    Mots-clés Pascal anglais : AIDS, Viral disease, Infection, HIV-1 virus, Human immunodeficiency virus, Lentivirinae, Retroviridae, Virus, HIV-2 virus, Evaluation, ELISA assay, Antibody, WHO, Human, South Africa, Africa, Immunopathology, Immune deficiency

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 95-0591666

    Code Inist : 002B06D01. Création : 01/03/1996.