The aim was to assess the significance of two biomarkers ; antibody to benzo (a) pyrene DNA adducts and concentration of hydroxyethylvaline haemoglobin adducts in samples from a well studied group of coke oven workers.
As a measure of exposure we have used 1-hydroxypyrene in urine.
Methods-Urine and blood samples were collected from coke oven workers and a control group.
Samples from coke oven plant workers were collected in January and June. 1-Hydroxypyrene was measured in urine by high performance liquid chromatography (HPLC), antibodies to benzo (a) pyrene DNA adducts were measured by ELISA and hydroxyethylvaline haemoglobin adducts were measured by gas chromatography-mass spectrometry (GC-MS).
Mean urinary 1-hydroxypyrene in samples from coke oven workers varied from 1.11 to 5.53 umol/mol creatinine and 0.14 umol/mol creatinine in the control group.
Workers at the top side had the highest values of urinary 1-hydroxypyrene.
Antibody to benzo (a) pyrene DNA adducts did not correlate with either 1-hydroxypyrene nor length of work at the coke oven plant.
But antibody concentration in samples collected in January was predictive of the concentration in samples collected in June.
Conclusion-1-Hydroxypyrene correlates well with exposure groups based on job description.
Antibodies to benzo (a) pyrene DNA adducts was related to people and not exposure.
Work at a coke oven plant might lead to increased hydroxyethylvaline haemoglobin adducts.
Mots-clés Pascal : Composé aromatique polycyclique, Exposition professionnelle, Four coke, Homme, Marqueur biologique, Sang, Plasma sanguin, Urine, Adduit moléculaire, Anticorps, Carcinogène, Médecine travail
Mots-clés Pascal anglais : Polycyclic aromatic compound, Occupational exposure, Coke oven, Human, Biological marker, Blood, Blood plasma, Urine, Molecular adduct, Antibody, Carcinogen, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0579841
Code Inist : 002B03L06. Création : 01/03/1996.