Monitoring of exposure to methylpentanes by diffusive sampling and urine analysis for alcoholic metabolites.
Objectives-To investigate the possibilities of personal ambient monitoring and biological monitoring for methylpentane isomers.
Methods-The performance of activated carbon cloth to absorb 2-and 3-methylpentane was studied by experimental vapour exposure followed by solvent extraction and gas chromatography (GC).
Urine from workers and rats exposed to 2-and 3-methylpentane was analysed by GC with or without acid or enzymatic hydrolysis.
Carbon cloth absorbed 2-and 3-methylpentane linearly to exposures up to eight hours and to 400 ppm, and was sensitive enough to detect a 15 minute peak of exposure.
The two isomers were clearly separated from hexane on a DB-1 column.
For analysis of the urine, enzymatic hydrolysis was superior to acid hydrolysis.
Exposure of rats to methylpentane vapours showed that 2-methyl-2-pentanol and 3-methyl-2-pentanol were excreted in urine in proportion to the dose of 2-methylpentane and 3-methylpentane, respectively. 2-Methyl derivatives of 1-3-and 4-propanol, 2-methylpentane-2,4-diol, and 3-methyl-2-pentanol were minor metabolites.
Analysis of urine from the exposed workers showed that 2-methyl-and 3-methyl-2-pentanol are leading urinary metabolites after exposure to the corresponding methylpentane.
Conclusions-Diffiusive sampling is applicable to monitor 2-and 3-methylpentane vapours as is the case for hexane vapour.
Mots-clés Pascal : Pentane dérivé, Solvant organique, Vapeur, Analyse quantitative, Lieu travail, Teneur air ambiant, Contrôle, Surveillance biologique, Urine, Marqueur biologique, Homme, Rat, Rodentia, Mammalia, Vertebrata, Animal, Exposition professionnelle, Médecine travail, Pentane(méthyl)
Mots-clés Pascal anglais : Pentane derivatives, Organic solvent, Vapor, Quantitative analysis, Work place, Ambient air concentration, Check, Biological monitoring, Urine, Biological marker, Human, Rat, Rodentia, Mammalia, Vertebrata, Animal, Occupational exposure, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0579615
Code Inist : 002B03L04. Création : 01/03/1996.