The investigation of Epstein-Barr viral sequences in 41 cases of Burkitt's lymphoma from Egypt : epidemiologic correlations.
Epstein-Barr virus (EBV) is associated with many human neoplasms, including Burkitt's lymphoma (BL).
Endemic BL in central Africa is more often EBV-associated than BL in the United States, where seroconversion for EBV occurs somewhat later than in Africa.
Therefore, the EBV association rate in BL may correlate more with the socioeconomic status of the population studied, which influences the age of EBV seroconversion, than with such factors as malaria, which may relate to the overall higher incidence rate in endemic regions.
Forty-one patients with BL in Egypt, which differs both climatically and racially from central African countries (i.e., Kenya, Uganda) where BL is endemic, were analyzed.
All biopsies were evaluated for EBV-encoded RNAs (EBER1) by RNA in situ hybridization, analyzed for p53 protein expression using the monoclonal antibody D07, and immunophenotyped using a panel of monoclonal antibodies that included L26 (CD20), Leu 22 (CD43), and A6 (CD45RO).
Twelve cases were evaluable for EBV subtype by polymerase chain reaction with EBV-specific primers.
The median age at diagnosis was 9 years (range, 2-22 years).
The biopsy site was extranodal in 29 patients and nodal in 12 patients.
All 41 cases were documented as B-cell neoplasms.
A hybridization signal for EBER1 RNA was identified in greater than 95% of the neoplastic cells in 30 of 41 cases (73%), whereas no signal was observed in 11 cases (27%).
Mots-clés Pascal : Lymphome Burkitt, Virose, Infection, Enfant, Homme, Adolescent, Exploration virologique, Virus Epstein Barr, Gammaherpesvirinae, Herpesviridae, Virus, Phénotype, Gène suppresseur tumeur, Expression génique, Epidémiologie, Egypte, Afrique, Hémopathie maligne, Lymphoprolifératif syndrome, Lymphome non hodgkinien, Exploration microbiologique, Gène p53
Mots-clés Pascal anglais : Burkitt lymphoma, Viral disease, Infection, Child, Human, Adolescent, Virological exploration, Epstein Barr virus, Gammaherpesvirinae, Herpesviridae, Virus, Phenotype, Tumor suppressor gene, Gene expression, Epidemiology, Egypt, Africa, Malignant hemopathy, Lymphoproliferative syndrome, Non Hodgkin lymphoma, Microbiological investigation, p53 gene
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0526216
Code Inist : 002B19B. Création : 01/03/1996.