We studied the molecular epidemiology of the recent fast-food restaurant chain-associated Escherichia coli O157 : H7 outbreak in Washington State.
Genomic DNAs prepared from strains isolated from 433 patients were probed with radiolabelled Shiga-like toxin (SLT) I and SLT II genes and bacteriophage lambda DNA and were subsequently analyzed for their restriction fragment length polymorphism (RFLP) patterns.
The SLT RFLP and lambda RFLP profiles of an E. coli O157 : H7 strain isolated from the incriminated beef and prototype patient were compared with those of the patient isolates for determination of the concordance between patterns.
Of the 377 patients with primary and secondary cases of infection epidemiologically linked to the outbreak, isolates from 367 (97.3%) of the patients displayed SLT RFLP and X RFLP profiles identical to those of the outbreak strains.
Isolates from 10 of the 377 (2.6%) patients possessed SLT RFLP and lambda RFLP profiles different from those of the outbreak strains, and the patients from whom those isolates were obtained were subsequently characterized as having non-outbreak-related infections.
The E. coli O157 : H7 strains isolated from 31 of 44 (70.4%) patients who were epidemiologically excluded from the outbreak were linked to the outbreak by RFLP typing.
Our results indicate that SLT RFLP and lambda RFLP analyses are stable and sensitive methods.
Mots-clés Pascal : Toxiinfection, Intoxication alimentaire, Epidémiologie moléculaire, Génotype, Gène, Bactériophage lambda, Siphoviridae, Bactériophage, Virus, Polymorphisme longueur fragment restriction, Epidémie, Homme, Colibacillose, Bactériose, Infection, Appareil digestif pathologie, Toxine, Restauration rapide, Toxine type Shiga
Mots-clés Pascal anglais : Toxiinfection, Food poisoning, Molecular epidemiology, Genotype, Gene, Phage lambda, Siphoviridae, Phage, Virus, Restriction fragment length polymorphism, Epidemic, Human, Colibacillosis, Bacteriosis, Infection, Digestive diseases, Toxin, Fast food, Shiga-like toxin
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0453400
Code Inist : 002A05B10. Création : 01/03/1996.