The effect of personal exposure to air pollution on DNA adducts in humans was analyzed in a group (n=30) of women working outdoors (up to 8 h/day) as postal workers or gardeners in the city of Teplice, Czech Republic (Northern Bohemia), where winter inversions may result in high levels of air pollution from coal combustion.
Ten of these women were followed up during the next winter season by repeated personal exposure monitoring and analysis of the DNA adducts in their white blood cells (in four sampling periods).
Personal exposure monitoring for respirable particles (<2.5 mum) was conducted for the 24 h period prior to collection of blood and urine.
Particle extracts were analyzed for carcinogenic polycyclic aromatic hydrocarbons (PAH).
Urine samples were collected for cotinine analysis to control for exposure to tobacco smoke.
DNA isolated from white blood cells was analyzed by 32P-postlabeling with the butanol enrichment procedure.
In the pilot study we found a significantly higher (P<0.05) level of DNA adducts in the 14 non-smoking women sampled on November 24, when their exposure to carcinogenic PAH was also significantly higher (P<0.05), compared with seven non-smoking women sampled on November 26.
We also found a significant correlation (r=0.541, P<0.016) between individual exposure to carcinogenic PAH and DNA adducts for the group of non-smokers (n=21).
No significant difference in DNA adduct levels was found between non-smokers and smokers.
Mots-clés Pascal : Carcinogène, Toxicité, Homme, DNA, Adduit moléculaire, Composé aromatique polycyclique, Pollution air, Surveillance biologique, Charbon, République tchèque, Europe, Leucocyte, Exposition professionnelle, Etude longitudinale, Epidemiologie, Marqueur biologique, Particule en suspension
Mots-clés Pascal anglais : Carcinogen, Toxicity, Human, DNA, Molecular adduct, Polycyclic aromatic compound, Air pollution, Biological monitoring, Coal, Czech Republic, Europe, Leukocyte, Occupational exposure
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0443207
Code Inist : 002B03M02. Création : 01/03/1996.