The nature of mutagenic burden due to occupational exposure to tobacco Rakes and dust was determined among 20 female tobacco processors (TP) and 20 matched controls (C) by testing urinary mutagenicity in the Ames assay.
In addition ; urinary cotinine was estimated as a marker of tobacco absorption.
Workers and controls were sub-divided into those with no tobacco habit (NH) and those habituated to the use of masheri (a pyrolysed form of tobacco) as a dentifrice (MH).
Cotinine was not detected in samples from C-NH while the mean urinary cotinine levels in TP-NH and TP-MH were significantly higher than that in C-MH (3.46±0.95 and 3.57±0.46 versus 1.80±0.58 mM/M creatinine ; P<0.02).
The majority of the urine samples from C-NH were non-mutagenic in the presence or absence of rat liver S9 while those from C-MH were mutagenic to TA98 and TA102 strains upon metabolic activation.
Generally, bêta-glucuronidase treatment reduced or abolished the mutagenic potential of workers'urine samples indicating that glucuronide conjugates may have partially contributed to direct mutagenicity.
Experiments using scavengers of reactive oxygen species revealed that direct mutagenicity in TA102 strain was mediated mainly via hydroxyl radicals.
The results clearly demonstrate that tobacco processors are exposed to a wide spectrum of mutagens that cause frame-shift, base pair substitution and oxidative damage.
Mots-clés Pascal : Mutagène, Homme, Femelle, Tabac sans fumée, Exposition professionnelle, Inde, Asie, Test mutagénicité, Urine, Cotinine, Bidi
Mots-clés Pascal anglais : Mutagen, Human, Female, Smokeless tobacco, Occupational exposure, India, Asia, Mutagenicity testing, Urine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0410917
Code Inist : 002B03E. Création : 01/03/1996.