The presence of human papillomavirus (HPV) sequences in 21 biopsies from cervical carcinomas, 11 specimens of tissues adjacent to tumours, 2 specimens of cervical tissues with radiation fibrosis from patients after radiation therapy of cervical cancer and 7 normal epithelial tissues from the patients with other genital tumours were examined by polymerase chain reaction (PCR) and Southern-blot analysis.
All tumours were HPV-positive by type-specific PCR and 86% by Southern-blot analysis.
In normal epithelial and adjacent tissues, HPV sequences were detected in 20% of samples by Southern-blot analysis and in 70% of samples by PCR, including 2 cases of tissues after radiation therapy.
HPV16 was the most prevalent type in tumours (18/21) as well as in normal epithelial tissues (5/7).
One HPV-positive tumour contained HPV18 DNA and 2 were doubly infected with HPVs 16 and 18 (2/21).
The persistence of exclusively episomal HPV16 DNA was observed in 5 out of 11 tumours examined : 3 cases of squamous-cell carcinomas on the early stage of tumour progression and 2 advanced tumours (squamous-cell carcinoma and adenocarcinoma).
The integration of HPV16 genome was detected in 6 out of 11 tumours, but most of them contained episomal forms of viral DNA simultaneously (5 out of 6).
The integrative HPV18 genome was found in 2 tumours examined, and the persistence of episomal forms was also observed in one of them.
Mots-clés Pascal : Epithélioma, Col utérus, Virose, Infection, Papillomavirus humain, Papillomavirus, Papovaviridae, Virus, Biologie moléculaire, Exploration, Prévalence, Epidémiologie, Homme, Femelle, Russie, Eurasie, Appareil génital femelle pathologie, Col utérus pathologie, Tumeur maligne
Mots-clés Pascal anglais : Carcinoma, Uterine cervix, Viral disease, Infection, Human papillomavirus, Papillomavirus, Papovaviridae, Virus, Molecular biology, Exploration, Prevalence, Epidemiology, Human, Female, Russia, Eurasia, Female genital diseases, Uterine cervix diseases, Malignant tumor
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0282890
Code Inist : 002B20C02. Création : 01/03/1996.