Mutations clustered in exon 5 of the p53 gene in primary nasopharyngeal carcinomas from Southeastern Asia.
Mutations in the p53 tumor suppressor gene play an important role in the development of many common human malignancies.
In nasopharyngeal carcinomas (NPC), p53 gene mutations were not detected in primary tumors, with one exception for a primary tumor displaying a p53 mutation at codon 280, whereas p53 mutations were identified in some metastatic and nude mouse-passaged NPC specimens.
In the present report, 41 NPC primary tumow of the undifferentiated carcinoma nasopharyngeal type (UCNT ; 21 from Hong Kong and 20 from Guangxi, southeastern China) were studied.
Four point mutations that result in amino acid substitutions were identified by PCR amplification of exons 2-9 and direct DNA sequencing, combined with PCR-single-strand conformation polymorphism analysis.
The 4 mutations detected were clustered within the DNA stretch from codon 175 to 177.
Our data, taken together with those of others, suggest that mutation in p53 may occur in NPC at various points during tumorigenesis.
Alternative mechanisms of p53 inactivation in NPC are also possible.
Mots-clés Pascal : Epithélioma, Nasopharynx, Carcinogenèse, Gène suppresseur tumeur, Mutation, Exon, Réaction chaîne polymérase, Exploration, Homme, Epidémiologie, Asie du sud est, Asie, ORL pathologie, Pharynx pathologie, Tumeur maligne, Biologie moléculaire, Polymorphisme conformation simple brin, Gène p53
Mots-clés Pascal anglais : Carcinoma, Nasopharynx, Carcinogenesis, Tumor suppressor gene, Mutation, Exon, Polymerase chain reaction, Exploration, Human, Epidemiology, South east Asia, Asia, ENT disease, Pharynx disease, Malignant tumor, Molecular biology, p53 gene
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0282251
Code Inist : 002B10B01. Création : 01/03/1996.