Hepatitis C virus (HCV) is a major cause of hepatitis among patients treated with maintenance hemodialysis.
Blood transfusion appears to be the primary risk factor, but nosocomial transmission of HCV in the dialytic environment has been hypothesized.
We addressed this issue by analyzing the individual variation of genomic sequences of HCV in 28 patients on chronic hemodialysis (HD) from the same department and 25 environmentally unrelated patients with HCV-related liver disease.
Genome variations of HCV were studied by single strand conformation polymorphism (SSCP) analysis of polymerase chain reaction products obtained from the 5'-untranslated region of the viral genome and by sequence analysis.
Six different SSCP patterns were identified in HD patients versus 16 in control patients.
Among HD patients the three more frequent SSCP patterns accounted for 85% of observations, while in the control group each pattern was found in 4 to 12% of patients.
The ability of SSCP to discriminate sequence variation was proven by sequence analysis which confirmed identity/diversity of sequences selected by SSCP.
The relative homogeneity of HCV variants in HD patients treated in the same HD and the high prevalence in this unit of a rare viral variant support the possibility of a nosocomial transmission of HCV in the dialytic environment.
Mots-clés Pascal : Hémodialyse, Homme, Service hospitalier, Hépatite virale C, Virose, Infection, Complication, Exploration virologique, Virus hépatite C, Flaviviridae, Virus, Séquence nucléotide, Variant, Epidémiologie, Milieu hospitalier, Epuration extrarénale, Appareil digestif pathologie, Foie pathologie, Exploration microbiologique, Biologie moléculaire, infection nosocomiale
Mots-clés Pascal anglais : Hemodialysis, Human, Hospital ward, Viral hepatitis C, Viral disease, Infection, Complication, Virological exploration, Hepatitis C virus, Flaviviridae, Virus, Nucleotide sequence, Variant, Epidemiology, Hospital environment, Extrarenal dialysis, Digestive diseases, Hepatic disease, Microbiological investigation, Molecular biology, Nosocomial infection
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0254967
Code Inist : 002B05C02G. Création : 01/03/1996.