A procedure based on the infection of CaCo-2 cells and molecular hybridization with specific cDNA probes has been developed forthe detection of infectious fastidious enteric viruses in environmental samples.
CaCo-2 cells, derived from a human colon adenocarcinoma, showed an increased sensitivity when compared to the usual routine host cell line to laboratory strains of rotavirus 3, reovirus 3, astrovirus 1, poliovirus 1, coxsackievirus A 24, enterovirus 70, and adenovirus 5,40, and 41.
Using this methodology, wild-type rotaviruses, enteric adenoviruses, enteroviruses, and for the first time, astroviruses have been detected in freshwater samples.
Direct dot-blot hybridization alone was not sufficient for virus detection from environmental samples.
CaCo-2 cells may be used as a universal in vivo amplification system for human enteric viruses, enabling the specific monitoring of infectious viral agents in the environment.
Mots-clés Pascal : Enterovirus, Picornaviridae, Virus, Rotavirus, Reoviridae, Adenoviridae, Homme, Eau usée, Détection, Méthode, Infection, In vitro, Hybridation moléculaire, DNA, RNA, Pollution eau, Qualité eau, Côlon, Lignée cellulaire, Milieu eau douce, Lignée CACO2
Mots-clés Pascal anglais : Enterovirus, Picornaviridae, Virus, Rotavirus, Reoviridae, Adenoviridae, Human, Waste water, Detection, Method, Infection, In vitro, Molecular hybridization, DNA, RNA, Water pollution, Water quality, Colon, Cell line, Freshwater environment
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0574206
Code Inist : 002A14C02. Création : 01/03/1996.