Evidence against heterozygous coagulation factor V 1691 G-A mutation with resistance to activated protein C being a risk factor for coronary artery disease and myocardial infarction.
The aim of our study was to determine the prevalence of the factor V mutation (position 1691 G-A) in patients with angiographically diagnosed coronary artery disease and myocardial infarction and, as a control, in blood donors.
This mutation has already been proved to be the main genetic risk factor for venous thrombosis.
In order to detect this mutation in exon 10 of the factor V gene we established a microtiter plate based hybridization assay for the specific detection of wild-type and mutant sequences in factor V gene segments, obtained after amplification by polymerase chain reaction.
This test enables us to screen a large number of samples.
The mutation was detected in 29 of 317 coronary artery disease (CAD) patients (9.1%) and 18 of 190 blood donors (9.5%) investigated.
The mean activated protein C resistance ratios were 3.18 and 3.11, with nearly identical distribution.
No increased prevalence of the factor V mutation was found in the CAD group.
In 10 of 29 CAD patients (35%) with the factor V 1691 G-A mutation and in 124 of 288 CAD patients without the mutation (43%) there was a history of myocardial infarction.
From our data we conclude that there is no increased risk of developing coronary atheroma or consecutive myocardial infarction resulting from the factor V mutation with protein C resistance.
Mots-clés Pascal : Infarctus, Myocarde, Cardiopathie coronaire, Facteur risque, Epidémiologie, Homme, Proaccélérine, Gène, Mutation, Protéine C, Etat activé, Appareil circulatoire pathologie, Myocarde pathologie, Facteur coagulation
Mots-clés Pascal anglais : Infarct, Myocardium, Coronary heart disease, Risk factor, Epidemiology, Human, Factor V, Gene, Mutation, Protein C, Activated state, Cardiovascular disease, Myocardial disease, Coagulation factor
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0571893
Code Inist : 002B12A03. Création : 01/03/1996.