Induction of nitric oxide synthase in a model of allergic occupational asthma.
We have studied the induction of nitric oxide during late allergic responses, using a guinea pig model of trimellitic anhydride (TMA) - induced airway allergy.
TMA is a low molecular weight chemical which can cause occupational asthma.
The activity of nitric oxide synthase (NOS) was investigated by the detection of 3H-labeled citrulline formation from 3H-labeled arginine.
In sensitized animals, challenge with TMA conjugated to guinea pig albumin (TMA-GPSA) increased the activity of Ca2+-independent NOS (inducible NOS ; iNOS) in lung and bronchial tissues at 15-17 h after challenge compared to nonsensitized animals.
The induction of iNOS activity was associated with an increased level of nitrite, an end metabolite of the 1-arginine-NO pathway, in bronchoalveolar lavage fluid.
In contrast to iNOS, the activity of Ca2+-dependent NOS (constitutive NOS ; cNOS) was not affected by the allergen challenge.
These results demonstrate that iNOS in bronchial tissue is induced late after allergen challenge in sensitized guinea pig.
Mots-clés Pascal : Asthme, Allergie, Exposition professionnelle, Anhydride organique, Azote monoxyde, Nitric-oxide synthase, Oxidoreductases, Enzyme, Modèle animal, Cobaye, Rodentia, Mammalia, Vertebrata, Animal, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Maladie professionnelle, Médecine travail
Mots-clés Pascal anglais : Asthma, Allergy, Occupational exposure, Organic anhydride, Nitrogen monoxide, Nitric-oxide synthase, Oxidoreductases, Enzyme, Animal model, Guinea pig, Rodentia, Mammalia, Vertebrata, Animal, Respiratory disease, Obstructive pulmonary disease, Occupational disease, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0568492
Code Inist : 002B06B. Création : 01/03/1996.