Polymerase chain reaction (PCR) - based screening methods were used to classify mutations arising in vivo at the hypoxanthine guanine phosphoribosyl-transferase (hprt) locus in small samples of human T-lymphocyte clones (<5X104 cells) from 29 bus maintenance workers exposed to diesel exhaust, and 14 control individuals.
All subjects were healthy, non-smoking males.
Among 462 T-cell mutants studied by multiplex-PCR of genomic DNA, only 12 (2.6%) deletions were found : three total deletions, five partial exon deletions and four mutants with one or two exons deleted.
Point mutations were classified in 323 mutants using reverse tanscriptase-PCR amplification : 74 (22.9%) of these had splice site mutations and 241 (74.6%) had coding errors.
Splice mutation was more frequent among the garage workers (24.8%) as compared to the controls (19.5%), possibly reflecting a polycyclic aromatic hydrocarbon-specific mutation induction in these workers.
Our results also show that both gene deletion and splice mutation at the hprt-locus in T-cells of healthy non-smokers could be less frequent than previously reported.
Mots-clés Pascal : Mutation ponctuelle, Hypoxanthine phosphoribosyltransferase, Pentosyltransferases, Glycosyltransferases, Transferases, Enzyme, Lymphocyte T, Clonage cellulaire, Homme, Exposition professionnelle, Maintenance, Autobus, Moteur diesel, Classification, Fréquence, Réaction chaîne polymérase, Délétion, Epissage, Toxicité, Mutagenèse, Garagiste
Mots-clés Pascal anglais : Point mutation, Hypoxanthine phosphoribosyltransferase, Pentosyltransferases, Glycosyltransferases, Transferases, Enzyme, T-Lymphocyte, Cell cloning, Human, Occupational exposure, Maintenance, Bus, Diesel engine, Classification, Frequency, Polymerase chain reaction, Deletion, Splicing, Toxicity, Mutagenesis
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0485718
Code Inist : 002B03N. Création : 01/03/1996.