In a prospective blinded controlled study, the efficacy of fluorescence in situ hybridization (FISH) to detect non-sperm male cells verifying sexual contact was examined.
Cervicovaginal smears (CVS) from 40 women with reported post-coital intervals were examined for sperm by cytology and for sperm and non-sperm male cells by FISH using X and Y chromosome specific DNA probes.
Fluorescence in situ hybridization identified sperm and/or non-sperm male cells in all specimens from women with positive coital histories, including when the partner had a vasectomy.
Male cells were also detectable by FISH in CVS up to 3 weeks after coitus.
In comparison, cytology identified sperm in 41% of the positive coital history cases, and none beyond 2 weeks.
Fluorescence in situ hybridization is highly sensitive and specific in detecting male cells, and can be performed rapidly on routine CVS.
Application of this technique can provide new and additional evidence of sexual contact when current tests are inconclusive.
Mots-clés Pascal : Cellule épithéliale, Mâle, Détection, Postcoïtal, Frottis vaginal, Exploration cytologique, Hybridation, In situ, Fluorescence, Sperme, Médecine légale, Evaluation performance, Technique, Homme, Abus sexuel, Biologie moléculaire
Mots-clés Pascal anglais : Epithelial cell, Male, Detection, Postcoital, Vaginal smear, Cytologic investigation, Hybridization, In situ, Fluorescence, Semen, Legal medicine, Performance evaluation, Technique, Human, Sexual abuse, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0456083
Code Inist : 002B30A10. Création : 01/03/1996.