Anti-benzo[a]pyrene diolepoxide-DNA adduct levels in peripheral mononuclear cells from coke oven workers and the enhancing effect of smoking.
The level of (±) - r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE) bound to DNA of lymphocytes plus monocytes in 39 coke oven workers exposed to polycyclic aromatic hydrocarbons (PAH) and 39 non-exposed persons (controls) were investigated, each of the groups consisting of smokers and non-smokers.
The adduct level was measured by an improved HPLC/fluorescence method (Rojas, M., Alexandrov, K., van Schooten, F.J., Hillebrand, M., Kriek, E. and Bartsch, H., Carcinogenesis, 15,557-560,1994) through the release of the corresponding benzo[a]pyrene (B[a]P) tetrols.
The anti-BPDE-DNA adduct was detected in 51% of coke oven workers exposed to PAH and in 18% of the non-exposed (control) subjects.
The mean level of antiBPDE-DNA adducts/108 nucleotides in coke oven workers (15.7 ± 37.8) was ~8 times higher than in non-exposed subjects (2.0 ± 8.7).
The interindividual variation of adduct levels was - 100-fold in coke oven workers and - 50-fold in controls respectively.
Smokers in the exposed group had 3.5 times more DNA adducts than non-smokers.
With the exception of one non-smoker with very high adduct levels (52.8 adducts/108), the control subjects showed the presence of barely detectable adducts in only 16% of the samples examined.
The increased in vivo formation in some smokers and high variability of anti-BPDE-DNA adducts in coke oven workers suggests variations in genetically controlled activation/inactivation reactions of PAH metabolism.
Mots-clés Pascal : Stéréoisomère anti, Benzo[a]pyrène-7,8-diol(9,10-époxy-7,8,9,10-tétrahydro), Carcinogène, Adduit moléculaire, DNA, Homme, Four coke, Mononucléaire, Monocyte, Lymphocyte, Sang, Exposition professionnelle, Tabagisme, Variant génétique, Comparaison interindividuelle, Benzo[a]pyrène
Mots-clés Pascal anglais : Anti stereoisomer, Carcinogen, Molecular adduct, DNA, Human, Coke oven, Mononuclear cell, Monocyte, Lymphocyte, Blood, Occupational exposure, Tobacco smoking, Genetic variant, Interindividual comparison
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0434653
Code Inist : 002B03L06. Création : 01/03/1996.