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  1. Mutations of the K-ras and p53 genes in gastric adenocarcinomas from a high-incidence region around Florence, Italy.

    Article - En anglais

    K-ras and p53 gene mutations in intestinal-type gastric carcinomas from a high-incidence area around Florence, Italy, were studied by single strand conformation polymorphism and DNA sequencing analysis.

    Single-strand conformation polymorphism analysis of K-ras indicated aberrant bands in 13 of 34 cases.

    Sequencing revealed point mutations in 7 (including two at a previously unreported site in codon 11), a significantly higher frequency than reported in countries other than Japan.

    No K-ras mutations were identified in stage III tumors.

    Single-strand conformation polymorphisms in p53 exons 5-8 occurred in 30 of 34 cases, with mutations identifiable by direct sequencing in 65% of the cases.

    Of these, 91% were base substitutions, a value similar to that usually reported, but the percentage of G : C to A : T transitions (90% in this study, 89% in all published European cases combined) differed significantly from that in Oriental cases (48%). The percentage of A : T to G : C transitions was greater in Oriental (22%) than European cases (2%), as was also true for transversions (30% in Oriental tumors, 9% in European tumors).

    The frequency of mutations at CpG sites (14%) varied significantly from the 67% in cases from a neighboring region in Italy.

    Helicobacter pylori infection was established in 19 cases and was somewhat more common in cases lacking a p53 mutation.

    Mots-clés Pascal : Epithélioma glandulaire, Estomac, Tumeur maligne, Pathogénie, Homme, Gène onc, Gène suppresseur tumeur, Mutation ponctuelle, Helicobacter pylori, Spirillaceae, Spirillales, Bactérie, Epidémiologie moléculaire, Estomac pathologie, Appareil digestif pathologie, Italie, Europe, Gène K-Ras, Gène ras, Gène p53

    Mots-clés Pascal anglais : Adenocarcinoma, Stomach, Malignant tumor, Pathogenesis, Human, Onc gene, Tumor suppressor gene, Point mutation, Helicobacter pylori, Spirillaceae, Spirillales, Bacteria, Molecular epidemiology, Gastric disease, Digestive diseases, Italy, Europe

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 95-0399895

    Code Inist : 002B13B01. Création : 01/03/1996.