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  1. Stability of blood (pro) vitamins during four years of storage at - 20°C : consequences for epidemiologic research.

    Article - En anglais

    We studied the effects of frozen storage on (pro) vitamin concentrations in EDTA-plasma and whole blood.

    Aliquots from 55 samples were analyzed before storage and after 3,6,12,24,36 and 48 months at - 20oC.

    Dramatic decreases occurred for EDTA-plasma concentrations of vitamin E between 6 and 12 months, vitamin A, total carotenoids and bêta-carotene after 1 year, and whole blood niacin.

    A smaller decrease was observed for folic acid at 1 year of storage, but the level remained constant thereafter.

    The vitamins D, B6, B12 (EDTA-plasma), B1 and B2 (whole blood) showed no decline during 4 years of storage.

    With the exception of folic acid, the observed decreases varied considerably among subjects.

    Therefore using EDTA-plasma stored longer than 1 year at - 20oC will result in highly attenuated odds ratios when assessing the relationship between vitamin A, carotenoids, or vitamin E with a given disease.

    Attenuation will also occur when using niacin concentrations in whole blood stored for 4 years at - 20oC.

    Mots-clés Pascal : Vitamine, Stabilité chimique, Sang, Conservation, Prélèvement, Homme, Méthode étude, Epidémiologie

    Mots-clés Pascal anglais : Vitamin, Chemical stability, Blood, Conservation, Samplings, Human, Investigation method, Epidemiology

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 95-0381468

    Code Inist : 002B30A01A1. Création : 01/03/1996.