Twenty-five asthmatic subjects with suspected alpha-amylase hypersensitivity were studied by skin-prick tests, a capture ELISA, immunoblotting and bronchial provocation tests, At the same time, different amylases were analysed by SDS-PAGE and immunoblotting using a polyclonal rabbit antiserum.
Eight patients showed a positive bronchial response to amylase.
Seven of them had positive skin-prick tests, with this method being the most sensitive approach for diagnosis.
However, in four cases, skin tests were also positive although the patients had a negative provocation test, thus demonstrating that skin tests are not specific.
ELISA and blotting showed similar results in terms of sensitivity and specificity.
The enzymes used by the workers included several antigens besides alpha-amylase.
The rabbit antiserum to alpha-amylase detected a protein in a wheat flour extract.
In one case, the IgE antibodies were specific only for a contaminant of lower molecular weight than amylase.
These facts suggest that proteins from the culture medium could be responsible for some cases of amylase hypersensitivity, making the diagnosis difficult.
The presence of amylase in another enzymatic extract, a protease produced by Aspergi/us oryzae, was proved by means of skin tests and immunoblotting, thus demonstrating the allergenic properties of this enzymatic preparation.
Mots-clés Pascal : Asthme, Hypersensibilité, alpha-Amylase, O-Glycosidases, Glycosidases, Hydrolases, Enzyme, Diagnostic, Exploration immunologique, Test cutané, Technique ELISA, Méthode immunoblotting, Test provocation, Etude comparative, Homme, Allergène, Réaction croisée, Exposition professionnelle, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Allergie, Immunopathologie, Boulangerie, Médecine travail
Mots-clés Pascal anglais : Asthma, Hypersensitivity, alpha-Amylase, O-Glycosidases, Glycosidases, Hydrolases, Enzyme, Diagnosis, Immunological investigation, Skin test, ELISA assay, Immunoblotting assay, Provocation test, Comparative study, Human, Allergen, Cross reaction, Occupational exposure, Respiratory disease, Obstructive pulmonary disease, Allergy, Immunopathology, Bakery, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0367223
Code Inist : 002B11B. Création : 01/03/1996.