Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine.
The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector.
A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used.
The sensitivity of detection was 1 pmol and high resolution (Rs=1.5) was reached.
We determined the D/L ratio of aspartic acid in dentin of 28 first premolars.
The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887 ; errors of ±1 year accounted for 46.4%, and no error exceeded 5 years.
Mots-clés Pascal : Médecine légale, Estimation, Age, Dentine, Dent, Analyse quantitative, Aspartique acide, Chromatographie HPLC, Homme, Mort
Mots-clés Pascal anglais : Legal medicine, Estimation, Age, Dentin, Tooth, Quantitative analysis, Aspartic acid, HPLC chromatography, Human, Death
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0350136
Code Inist : 002B30A10. Création : 01/03/1996.