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  1. Age estimation using a modified HPLC determination of ratio of aspartic acid in dentin.

    Article - En anglais

    Diastereomeric dipeptides were derived from the amino acid enantiomers in dentin by O-phthalaldehyde-N-acetyl-L-cysteine.

    The products were separated using high performance liquid chromatography (HPLC) and detected by fluorescence detector.

    A short analysis time (total analysis time was 15 min, including retention time, sample derivatization time and column regeneration time) was used.

    The sensitivity of detection was 1 pmol and high resolution (Rs=1.5) was reached.

    We determined the D/L ratio of aspartic acid in dentin of 28 first premolars.

    The correlation value between the D/L ratio of aspartic acid and actual age was 0.9887 ; errors of ±1 year accounted for 46.4%, and no error exceeded 5 years.

    Mots-clés Pascal : Médecine légale, Estimation, Age, Dentine, Dent, Analyse quantitative, Aspartique acide, Chromatographie HPLC, Homme, Mort

    Mots-clés Pascal anglais : Legal medicine, Estimation, Age, Dentin, Tooth, Quantitative analysis, Aspartic acid, HPLC chromatography, Human, Death

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 95-0350136

    Code Inist : 002B30A10. Création : 01/03/1996.