Epstein-Barr virus (EBV) type B, a less potent transformer of B lymphocytes than type A, has rarely been detected in EBV-associated neoplasms except in AIDS-related lymphomas, in which about 50% of the cases contained this sub-type.
In this study we analyzed the association of EBV and the distribution of virus sub-types in Asian non-Hodgkin's lymphoma (NHL) of the upper aerodigestive tract.
We studied archival material of 29 NHL cases from Malaysia.
B-and T-cell associated antigens were demonstrated by immunohistochemistry, and EBV early RNA EBER-I was demonstrated using the RNA in situ hybridization technique.
EBV was detected in the majority of tumour cells in 11/13 T-NHL but in only 1/16 B-NHL.
EBV was sub-typed by single-step polymerase chain reaction of the EBNA-2 gene.
This was successful in 9/10 cases of EBER-1-positive tumours and all contained type-A virus only.
Our results showed a preponderance of T-cell lymphoma of the upper aerodigestive tract in the ethnic Chinese group of Malaysian patients, and EBV was strongly associated with T-NHL but not with B-NHL.
Our results suggest that type-A EBV is the prevalent sub-type in Asian NHL of the upper aerodigestive tract, similarly to findings in Asian nasopharyngeal carcinoma.
Mots-clés Pascal : Lymphome, Voie aérodigestive supérieure, Homme, Exploration virologique, Virus Epstein Barr, Gammaherpesvirinae, Herpesviridae, Virus, Virose, Infection, Incidence, Epidémiologie, Immunocytochimie, Biologie moléculaire, Malaisie, Asie, Hémopathie maligne, Lymphoprolifératif syndrome, ORL pathologie, Pharynx pathologie, Exploration microbiologique, Type histologique
Mots-clés Pascal anglais : Lymphoma, Upper aerodigestive tract, Human, Virological exploration, Epstein Barr virus, Gammaherpesvirinae, Herpesviridae, Virus, Viral disease, Infection, Incidence, Epidemiology, Immunocytochemistry, Molecular biology, Malaysia, Asia, Malignant hemopathy, Lymphoproliferative syndrome, ENT disease, Pharynx disease, Microbiological investigation, Histological type
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 95-0282888
Code Inist : 002B19B. Création : 01/03/1996.